Bioremediation Tofu Liquid Waste Based on Chemical Oxygen Demand (COD) Parameters

Background and Objective: The majority of liquid waste generated by the tofu industry in Semarang exceeds recommended limit. This condition disturbs ecology surrounding environment. so a solution is needed to manage it. use bacteria bioremediation technology has been proven reduce pollutants produced industry. purpose this study was identify specific test their ability bioremediate from wastewater using Chemical Oxygen Demand (COD) parameter.
 Methodology: exploratory experimental nature, with variable degradative degrading industrial based on COD parameters. Bacterial identification performed 16S-rRNA Gene Analysis method, genomic DNA extraction Quick-DNA Miniprep Kit, PCR amplification, bi-directional sequencing. 
 Results: results selected show that they are molecules type nucleic acid query length 1394. lineage report 93% phylogenetic tree as Bacillus subtilis, Bioremediation shows can be degraded. degradation percentage 17%.
 Conclusion: subtillis, bacterium degrades wastewater, found an indigenous bacteria.